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J Proteomics. 2012 Jul 19;75(14):4555-69.
Identifcation and quantifcation of concentration-
dependent biomarkers in MCF-7/BOS cells
exposedto17β-estradiol by2-DDIGEandlabel-free
proteomics.
Collodoro M, Lemaire P, Eppe G, Bertrand V, Dobson R, Mazzucchelli G, Widart J, De Pauw E,
De Pauw-Gillet MC.
Abstract
This paper reports the identifcation of biomarkers resulting from the exposure of MCF-7/
BOS cells to 17β-estradiol (E(2)). The biomarkers were identifed using 2 independent and
complementary techniques, 2-D DIGE/MALDI-TOF peptide mass fngerprint, and 2-D UPLC-
ESI MS/MS. They were identifed from the cytosolic fractions of cells treated for 24h with
mitogenic concentrations of 1, 30 and 500 pM of 17β-estradiol. Five biomarkers were up-
regulated proteins, namely HSP 74, EF2, FKBP4, EF1 and GDIB and one was a down-regulated
protein, namely K2C8. Three of these proteins, EF2, FKBP4 and K2C8 are implicated in a
network centered on the estrogen receptors ESR1 and ESR2 as well as on AKT1. After the
discovery phase, three biomarkers were selected to test the presence of estrogens using
selected reaction monitoring (SRM). They were monitored using SRM after incubation of MCF-
7/BOS in the presence of E(2) for confrmation or selected xenoestrogens. Daidzein, coumes-
trol and enterolactone induced an up-regulation of EF2 and FKPB4 proteins, while tamoxifen
and resveratrol induced a down-regulation. The exposure of all phytoestrogens induced the
down-regulation of K2C8. These markers form a preliminary molecular signature that can be
used when testing the estrogenic activity of xenobiotics, either pure or in mixtures.
Biofouling. 2012;28(4):395-404.
Biomolecule-based antibacterial coating on a
stainless steel surface: multilayer flm build-up
optimization and stability study.
Vreuls C, Zocchi G, Vandegaart H, Faure E, Detrembleur C, Duwez AS, Martial J,
Van De Weerdt C.
Abstract
The goal of this paper was to establish the durability profle of antibacterial multilayer thin
flms under storage and usage conditions. Thin flms were built on stainless steel (SS) by means
of a layer-by-layer process alternating a negatively charged polyelectrolyte, polyacrylic acid,
with a cationic antibacterial peptide, nisin. SS coupons coated with the antibacterial flmwere
challenged under environmental and usage conditions likely to be encountered in real-world
applications. The change in antibacterial activity elicited by the challenge was used as an
indicator of multilayer flm resistance. Antibacterial SS samples could be stored for several
weeks at 4°C in ambient air and antibacterial flms were resistant to dipping and mild wiping
in water and neutral detergent. The multilayer coating showed some weaknesses, however,
that need to be addressed.