57
1294
Confocal analyses (on Leica SP2, Leica
SP5, Nikon A1R and Olympus FV1000
50
Confocal Time lapses (on Nikon A1R
and Olympus FV1000)
272
Epifuorecence analyses (on Olympus
FSX100, Olympus CKX-41 and Nikon
E800)
1305
Flow cytometry analyses (CBA, cell
viability, cell phenotyping, cell cycle,
cell proliferation, DNA damage)
237
Cell sorting (FACS Vantage, FACS
AriaIIIu 4L SORP)
18
High Content analyses (on BD
Pathway 855)
216
Users both from the academic or
private sector
Examples of publications including
results partly obtained thanks to the
GIGA-Imaging and Flow Cytometry
platform
Close P, Gillard M, Ladang A, Jiang Z, Papuga J,
Hawkes N, Nguyen L, Chapelle JP, Bouillenne F,
Svejstrup J, Fillet M, Chariot A. DERP6 (ELP5)
and C3ORF75 (ELP6) Regulate Tumorigenicity
and Migration of Melanoma Cells as Subunits
of Elongator. J Biol Chem 2012;287:32535-
45.
Lecomte J, Masset A, Blacher S, Maertens L,
Gothot A, Delgaudine M, Bruyere F, Carnet
O, Paupert J, Illemann M, Foidart JM, Lund IK,
Hoyer-Hansen G, Noel A. Bone marrow-de-
rived myofbroblasts are the providers of
pro-invasive matrix metalloproteinase 13 in
primary tumor. Neoplasia 2012;14:943-51.
Manfroid I, Ghaye A, Naye F, Detry N, Palm S,
Pan L, Ma TP, Huang W, Rovira M, Martial JA,
Parsons MJ, Moens CB, Voz ML, Peers B. Ze-
brafsh sox9b is crucial for hepatopancreatic
duct development and pancreatic endocrine
cell regeneration. Dev Biol 2012;366:268-78.
Théâtre E, Frederix K, Guilmain W, Delierneux
C, Lecut C, Bettendorf L, Bours V, Oury C.
Overexpression of CD39 in mouse airways
promotes bacteria-induced infammation.
J Immunol 2012;189:1966-74.
Imaging and Flow Cytometry
Among the technologies developed within the GIGA, confocal imaging and fow cytometry have
become, over the last decade, really essential for most biomedical research programs. Quite complex
technologies, they allow the fast and accurate study of molecules at the cellular level. Through detec-
tion using fuorochrome-coupled molecules emitting at diferent wavelengths, confocal microscopy can
highlight simultaneously a large number of fuorochromes and can study extremely precisely intracellular
localization. Thanks to an thermostatized chamber built on the microscopes, the cells can be maintained
in optimal experimental conditions, which allows to record sequential images and track over time the
subcellular localization of a protein of interest or cells behavior in response to a stimulus or to given
experimental conditions.
Flow cytometry allows qualitative and quantitative analysis of particles, for example monodisper-
sed cells, previously marked with fuorescent probes targeting molecules very diverse like membrane
antigens, cytokines, nucleic acids, viral receptors, calcium ions .... This technique, which allows the analysis
of blood cells, cells isolated from tissue or from a cell line or any particles larger than one micron (plate-
lets, bacteria, yeast ...), is an essential tool, not only for the simultaneous detection of several molecules of
interest, but also for the study of cell cycle, cell ploidy, cell proliferation, DNA damage or cell viability.
Recent developments using microbeads specifcally recognizing soluble molecules allow to detect and
quantify from biological fuids or culture media, molecules such as immunoglobulins or cytokines involved
in the infammatory response and signaling pathways. Sorter fow cytometers can also clone cells or sort
simultaneously, under sterile conditions, up to 4 cell populations.
Technologies such as high throughput imaging, laser microdissection, intravital imaging or circulating tumor
cells analysing are also available in the Imaging and Flow Cytometry platform.
Contacts
Sandra Ormenese
Gustavo Moraes
Raafat Stephan
imaging.giga@ulg.ac.be
www.giga.ulg.ac.be/imaging