68 GIGA 2021 Annual Report www. gigacellimaging. uliege.be www. gigaflowcytometry. uliege.be KEY FIGURES 2635 Confocal acquisitions 2424 Flow cytometry analyses 474 FACS cell sorting 240 High content screening analyses 344 Users 75 Research groups (73 ULiège/2 external) 8 companies 174 Training courses given on the various instruments SOME PRACTICAL IMPLEMENTATIONS LABORATORY OF IMMUNOPHYSIOLOGY, GIGA Thomas Marichal and his team are interested in how innate immune cells like neutrophils behave in the lung tissue during inflammation. An important function of neutrophils relies on their ability to release Neutrophil Extracellular Traps (NETs). Detecting and quantify NETs components (e.g., DNA or granule/histone proteins) can be very difficult in human samples. In this collaboration, the GIGA Cell Imaging platform suggested a method to stain the formalin-fixed paraffin-embedded (FFPE) tissue with a fluorescent DNA dye and antibodies against myeloperoxidase (MPO) and citrullinated histone 3 (Cit-H3). The platform also provided a standardized method for NETs volume quantification based on the measurement of the volume of structures exhibiting co-localization of Cit-H3 and MPO outside the cell. Such a technique not only allows the unambiguous identification of NETs in tissue sections, but also their quantitation and relationship with surrounding tissues. This collaboration led to the following publication: Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections. Radermecker C., Hego A., Delvenne P. and Marichal P., Bio Protoc (2021) 11 (18). TEAM Raafat Stephan Flow Cytometry Expert Céline Vanwinge Flow Cytometry Expert Alexandre Hego Cell Imaging & Image Analysis Expert Gaëtan Lefevre Cell Imaging Expert MANAGER Sandra ORMENESE sandra.ormenese@ uliege.be GIGA-CELL IMAGING & FLOW CYTOMETRY
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