GIGA 2021 Annual Report 79 LABORATORY OF TRANSLATIONAL GASTROENTEROLOGY A study done by the Translational Gastroenterology laboratory of Prof. Edouard Louis aimed at identifying biomarker candidates to distinguish dysplasic tissue from inflamed and normal colon tissues. In this collaboration, the role of the platform was to perform the shotgun proteomics analysis of small tissue section collected using laser microdissection (GIGA-Imaging platform). Using optimized sample preparation and LC-MS/MS methods developed and standardized by the GIGA-Proteomics platform and MSLab teams, this study lead to the identification of a specific biomarker candidate that was subsequently validated using immunohistochemistry. This collaboration led to the following publication: Solute Carrier Family 12 Member 2 as a Proteomic and Histological Biomarker of Dysplasia and Neoplasia in Ulcerative Colitis, Merli AM, Vieujean S, Massot C, Blétard N, Quesada Calvo F, Baiwir D Mazzucchelli G, Servais L, Wéra O, Oury C, De Leval L, Sempoux C, Manzini R, Bluemel S, Scharl M, Rogler G De Pauw E, Coimbra Marques C, Colard A, Vijverman A, Delvenne P, Louis E, Meuwis MA. J Crohns Colitis. 2020 Sep 13;jjaa168. SELECTED PUBLICATIONS Wobble tRNA modification and hydrophilicamino acid patterns dictate protein fate. Rapino F, Zhou Z, Roncero Sanchez AM, Joiret M, Seca C, El Hachem N, Valenti G, Latini S, Shostak K, Geris L, Li P, Huang G, Mazzucchelli G, Baiwir D, Desmet CJ, Chariot A, Georges M, Close P. Nat Commun. 2021 Apr 15;12(1):2170. Characterization of the human eccrine sweat proteome—a focus on the biological variability of individual sweat protein profiles. Burat B, Reynaerts A, Baiwir D, Fléron M, Eppe G, Leal T, Mazzucchelli G. Int J Mol Sci. 2021 Oct 8;22(19):10871. Liquid chromatography setup-dependent artefactual methionine oxidation of peptides: The importance of an adapted quality control process. Baumans F, Hanozin E, Baiwir D, Decroo C, Wattiez R, De Pauw E, Eppe G, Mazzucchelli G. J Chromatogr A. 2021 Sep 27;1654:462449. In vivo N-Terminomics Highlights Novel Functions of ADAMTS2 and ADAMTS14 in Skin Collagen Matrix Building. Leduc C, Dupont L, Joannes L, Monseur C, Baiwir D, Mazzucchelli G, Deroanne C, Colige A, Bekhouche M. Front Mol Biosci. 2021 Mar 19;8:643178.
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