platforms 86 platforms 87 Platform Proteomics GIGA-Proteomics offers a wide range of services that include mass determination, identification, and relative quantification of thousands of proteins from various sources such as biopsies, cell extracts, sorted cells, or laser microdissected FFPE tissues for differential label-free proteomics even starting from a low number of cells. The platform offers access to advanced technologies such as liquid chromatography, mass spectrometry, and bioinformatics tools to provide reliable and accurate results for proteomics analyses. Left to right : Maximilien Fleron, Gabriel Mazzucchelli, Dominique Baiwir, Nancy Rosiere, Lisette Trzpiot In February 2023, the novel mass spectrometer dedicated to proteomics analysis at the (single-) cell level, timsTOF SCP, was installed thanks to a grant from the Belgian foundation against cancer (Fondation contre le Cancer, grant awarded to Pr. Agnès Noël). The sample preparation, already developed taking advantage of the synergy between the GIGA-Proteomics platform and the ChipOmics project of MSLab (Win2WAL) have been combined with the sensitivity of the new mass spectrometer. This allows performing proteomics analysis starting from only 100 cells sorted using the FACS of the Flow Cytometry Platform. Within the ImmunAID european project (Immunome project consortium for AutoInflammatory Disorders), aiming at improving the diagnosis for some systemic autoinflammatory diseases now relying only on clinical symptoms, the GIGA-Proteomics platform is performing the differential proteomics analyses using liquid chromatography hyphenated to mass spectrometry with the laboratory of Rhumatology (Dr. Dominique De Seny). In 2023, 186 plasma samples have been depleted and analysed by 2D-LC-MS/MS for this project. In 2024, the new technology of LC-MS acquisition allowed by the timsTOF SCP will also be optimized to shorten the analysis time for samples that are not limited by available amount of material. We also plan to implement a technology switch for all samples submitted for differential proteomics to ensure consistent or even improved quality of results for researchers, particularly for some model organisms, at lower costs due to shorter acquisition times. In 2024, we will introduce sweat proteomics, a new and promising biological matrix. The platform will support the development of a systematic laser-based microdissection sampling of tissue slices with a lateral resolution of 25 µm, followed by shotgun proteomics, enabling the identification of thousands of proteins per pixel. In 2023 Dominique Baiwir & Gabriel Mazzucchelli, GIGA-Proteomics Managers D.Baiwir@uliege.be gabriel.mazzucchelli@uliege.be www.gigaproteomics.uliege.be Contact
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